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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Mol Carcinog. 2019 Apr 24;58(8):1400–1409. doi: 10.1002/mc.23023

Fig. 4. IR induces CELF2 and autophagy.

Fig. 4.

(A) Expression of CELF2, Beclin-1, Atg5, and Atg12 mRNA in CRC cell lines (HCT116 and SW480) with 0 or 6 Gy IR. Graph depicts cumulative results of two biological repeats plated in triplicate.

(B) Representative immunoblot of HCT116 or SW480 with 0 or 6 Gy IR demonstrates increased expression of CELF2, Beclin-1, Atg5, and Atg12 in irradiated cells.

(C) Immunofluorescence of LC3 puncta (green) in CRC cells (nuclei stained blue with hoescht stain) treated with CQ (80 μM for 2 hours). Graphs represent cumulative results from duplicate experiments of at least 30 cells counted per group. Error bars represent ± SEM.