Fig. 7.

Detection of PS+ TF+ EVs using SP-PLA and lactadherin capture. ( A ) Relipidated TF was spiked into buffer or EV-free plasma and detected with SP-PLA after lactadherin capture. Levels relative to control with EV-free plasma (set to 1) are shown. ( B ) Relipidated TF was spiked at different concentrations in PLA buffer and TF+ EVs were detected with SP-PLA after lactadherin capture. Error bars represent standard deviation from technical replicates ( n  = 2). ( C ) TF PCA of relipidated TF was measured using a direct activity assay after capture directly in the buffer or after lactadherin capture.