Fig. 1. Effect of IL-32γ on CSC formation.

a Flow-cytometric analysis demonstrates the percentage of tumorigenic CD133+ A549 cells in histogram (CD133-FITC) at the FL1 channel. b CD133+/– isolated cells were cultured for 9 days in a six-well plate, which showed self-renewal capacity and tumor-sphere formation efficiency (after sphere formations, cells were stained with Crystal Violet) and photographed. c CD133+ A549 CSC cells labeled with the nuclear stain DAPI (blue) and anti-CD133 directly conjugated to PE (red). After staining, cells were observed by fluorescent microscopy for expression and location of CD133. d CD133+ A549 CSCs were transfected with IL-32γ or control vector (1 µg/well; six-well plate) for 48 h after seeding, and analyzed by anti-IL-32 (6×Myc-tagged IL-32γ), anti-CD133, anti-ALDH1A1, anti-Sox2, and anti-Oct4 western blotting