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. 2019 Jul 1;10(7):506. doi: 10.1038/s41419-019-1737-4

Fig. 7. Effect of IL-32γ on ITGAV-mediated STAT5 pathway.

Fig. 7

a CD133+ A549 cells were transfected with ITGAV siRNA (100 nM) for 24 h and then the expression of pSTAT5, pERK, CD133, and cleaved caspase-3 was determined by western blotting. b CD133+ A549 cells were transfected with IL-32γ for 24 h and then transfected with ITGAV plasmid for another 24 h. After that, the expression of pERK, pSTAT5, and cleaved caspase-3 was determined by western blotting. c CD133+ A549 cells were transfected with IL-32γ for 24 h and then transfected with ITGAV siRNA (100 nM) for another 24 h. After that, the expression of pSTAT5, pERK, CD133, and cleaved caspase-3 was determined by western blotting. d CD133+ A549 cells were transfected with IL-32γ for 24 h, and then transfected with ITGAV siRNA (100 nM) for another 24 h and cultured for 9 days in a six-well plate, which showed self-renewal capacity and tumor-sphere formation efficiency (after sphere formations, cells were stained with Crystal Violet), and photographed at 20× resolution. n = 5, *p < 0.05 (control vs. IL-32γ or ITGAV siRNA); #p < 0.05 (ITGAV siRNA vs. IL-32γ + ITGAV siRNA)