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. 2019 Jul 1;9:9451. doi: 10.1038/s41598-019-46013-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tissue specificity of 7-ethoxycoumarin deethylation activity in silkworm. (A) The midgut, fat body, silk gland, and Malpighian tubule were extirpated from silkworms and incubated in medium containing 7-ethoxycoumarin at 30 °C. Medium was collected, extracted by acetone, and evaporated. Samples discovered in 50 mM NaOAc buffer (pH 6.0) were treated with beta-glucosidase. The amount of 7-hydroxycomarin, a metabolite, was determined by fluorometry. (B) Inhibition of 7-ethoxycoumarin deethylation by cimetidine in the silkworm midgut. Silkworm midgut was incubated in medium containing 7-ethoxycoumarin and cimetidine at 30 °C. Samples were collected, extracted by acetone, and treated with beta-glucosidase. The amount of 7-hydroxycomarin was determined by fluorometry.