Skip to main content
. 2019 Jul 1;10(7):510. doi: 10.1038/s41419-019-1701-3

Fig. 2. Knockout of Nrf2 leads to the degeneration of invertebral discs (IVDs) during aging.

Fig. 2

a RNA level of Nrf2 in 3, 6, 9, and 12-month-old mice (n = 5, respectively), assayed by real-time PCR. The relative unit of Nrf2 expression for 3-month-old mice was set to 1. *P < 0.05. b Protein level of Nrf2 in IVD of 3, 6, 9, and 12-month-old mice (n = 5, respectively), assayed by western blotting. c Histological degenerative scores of IVDs in Nrf2+/+ and Nrf2−/− mice aged 3 months and 9 months. Histological changes were observed by hematoxylin and eosin (HE). d Analysis of the proteoglycan extracellular matrix composition by Safranin O staining of sections from the intervertebral discs (IVDs) in Nrf2+/+ and Nrf2−/− mice aged 3 months and 9 months