Fig. 2. Hypoxia-induced ZEB1 in cancer cells enhances TAM migration in vitro.

a Immunofluorescence revealed that hypoxic SiHa and C33a cells exhibited upregulated expression of CAIX (green) and ZEB1 (red). Nuclei were stained with DAPI (blue) (scale bar, 50 μm). b Western blotting shows that the protein levels of both ZEB1 (upper panel) and CAIX (middle panel) were higher in hypoxic cancer cells than in normoxic cancer cells (left panel shows SiHa cells; right panel shows C33a cells). c The effect of conditioned media (CM) from hypoxia-treated cervical cancer cells on THP-1 cells was detected by transwell migration assay. Scale bar, 50 μm. *P < 0.05 by Student’s t-test