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. 2019 Jul 1;10(7):508. doi: 10.1038/s41419-019-1748-1

Fig. 4. CCL8–CCR2 interaction mediates TAM infiltration under hypoxic conditions.

Fig. 4

a Using a migration test, we identified that the migration effect of hypoxic cervical cancer cells on THP-1 cells was almost equivalent to that of 100 ng/ml CCL8. Bindarit (a CCL8 synthesis inhibitor) and siCCR2 significantly impaired the directional migration of THP-1 cells. Scale bar, 100 μm. b Immunofluorescence staining revealed that the level of CCR2 expression was increased in THP-1 cells incubated with 100 ng/ml CCL8 and decreased in THP-1 cells treated with bindarit or siCCR2. Scale bar, 100 μm. c Statistical analysis showing the number of migrated THP-1 cells. d Statistical analysis showing the number of migrated RAW264.7 cells. e Statistical analysis showing the fold change of the mean fluorescence intensity (MFI) of CCR2 in THP-1 cells. f Statistical analysis showing the fold change of the MFI of CCR2 in RAW264.7 cells