Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 1;10:2898. doi: 10.1038/s41467-019-10899-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Vaccine-induced antibodies neutralize tier 2 viruses. a Summary of the vaccine regimens and macaque demographics from which the three antibodies DH727.2, DH753, and DH796.1 were isolated. b Antigen-specific single B cell sorting of vaccinated macaque PBMCs. Antigen-specific single B cells were isolated with fluorophore-labeled CH505 gp120 or CON-S gp140 as indicated on the x and y-axes. The B cell encoding the antibody of interest is shown as a colored square in the FACS plot. c, d In vitro antibody neutralization of HIV-1 infection of TZM-bl cells. Each symbol signifies one of the 292 HIV-1 isolates tested. Neutralization titers are represented as the concentration in µg mL^‒1 that inhibits c 50% or d 80% of virus replication (IC50 or IC80 respectively). SIVmac239 and MuLV were used as negative controls and titers for both viruses were > 50 µg mL^‒1. Horizontal bar indicates the geometric mean neutralization titer of all isolates. The neutralization breadth is shown above each column as the percentage of viruses neutralized by 50% c or 80% d. e Maximum percent neutralization achieved by each vaccine-elicited antibody against 93 early/acute tier 2 clade C HIV-1 isolates. Each symbol signifies one HIV-1 isolate. Horizontal bar indicates the mean maximum percent neutralization. f, g Vaccine-induced antibodies preferentially neutralize the most sensitive tier 2 viruses. Polyclonal plasmas from HIV-1 infected individuals were used to phenotype 101 HIV-1 isolates as tier 2 viruses f. The plasma dilution that inhibited 50% of virus replication (ID50) for each virus is shown for all serum samples as the geometric mean titer (GMT). The viruses are ordered according to increasing sensitivity to the polyclonal sera neutralizing antibodies. g Vaccine-induced antibodies DH727.2 (red), DH753 (blue), and DH796.1 (green) neutralization titers as IC50 in µg mL^‒1 for the 101 tier 2 HIV-1 isolates shown in f. h Venn diagram showing the overlap of viruses from the 12-virus global panel that were neutralized by DH727.2, DH753, DH796.1 isolated from vaccination and macaque immune sera from monkeys immunized with BG505 SOSIPs²⁹. Five of the viruses were not neutralized by DH727.2, DH753, DH796.1 or BG505 SOSIP-immune sera. Source data are provided as a Source Data file