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. 2019 Jul 1;9:9474. doi: 10.1038/s41598-019-45905-6

Table 2.

Multi-gradient elution program used for purification of C-PC by ion exchange chromatography method.

Time(min) Buffer A (%) Buffer B (%)
0 95 5
30 95 5
130 85 15
270 83 17
335 70 30
400 0 100