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. 2019 Jul 1;9:9467. doi: 10.1038/s41598-019-45768-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The wild-type laboratory strain was centrifuged and separated into supernatant and pellet fractions. Each fraction was treated with 50 μM of gGlu-HMRG with or without an inhibitor of γ-glutamyltranspeptidase (GGT). Addition of the GGT inhibitor suppressed green fluorescence by the wild-type strain. Fluorescence was observed in both fractions, albeit at a greater intensity in the former.