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. 2019 Jul 1;9:9437. doi: 10.1038/s41598-019-46019-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Iron efflux from Cp KO astrocytes and Heph KO oligodendrocytes. Non-heme iron concentrations in WT and Cp KO astrocytes (A), and WT and Heph KO oligodendrocytes (B) at 0, 12, 24 hours after the cells had been treated with iron then transferred to fresh medium without added iron. Iron within the cells at the 0 hour time point was set to 100%. Data are representative of at least three independent experiments. Values are presented as the means ± SEMs; *P < 0.05, **P < 0.01, different from WT, as determined by unpaired Student’s t-test.