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. 2019 Jul 1;10:2901. doi: 10.1038/s41467-019-10681-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Acute c-Src ablation diminishes both OXPHOS and glycolysis. a ErbB2 + murine breast cancer cells with wild-type (c-Src^+/+) or conditional (c-Src^L/L) Src alleles were infected with adenoviruses bearing Cre recombinase or LacZ. Immunoblots show c-Src expression, mTORC1 and AMPK signaling and PRC2 component expression at 96 h post-infection. Data are representative of 3 cell lines per genotype. b LC/MS analysis of AMP:ATP ratios in control (LacZ) and Cre-expressing cell lines (n = 3 cell lines per genotype). c Basal, maximal (FCCP), ATP-synthesis-coupled (Oligomycin A), and non-mitochondrial (rotenone/antimycin A) oxygen consumption rates (OCRs) of ErbB2-expressing c-Src^+/+ and c-Src^L/L breast cancer cell lines (n = 2 per genotype) infected with Cre or LacZ adenoviruses. Representative of 2 cell lines per genotype. d Basal OCR and ECAR of c-Src^+/+ and c-Src^L/L cell lines infected with Cre adenoviruses, expressed as a percentage of the rate measured in the LacZ control for each cell line. e Glucose consumption and lactate levels in conditioned media from c-Src^+/+ and c-Src^L/L cell lines infected with Cre adenoviruses, expressed as a percentage of levels observed in LacZ-expressing controls. All bar charts show mean ±SEM, with * = p < 0.05 and ** = p < 0.01 (unpaired, two-tailed Student’s t-test)