Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 1;10:2901. doi: 10.1038/s41467-019-10681-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — Ezh2 is required for ErbB2-driven mammary epithelial transformation. a Kaplan–Meier analysis of mammary tumor onset in MMTV-NIC mice with wild-type Ezh2 alleles (NIC/Ezh2^+/+) and mice heterozygous (NIC/Ezh2^+/L) or homozygous (NIC/Ezh2^L/L) for the conditional Ezh2 allele. Inset table shows group sizes (n), average time to tumor onset and penetrance of mammary tumors. b Immunofluorescence (IF) analysis of ErbB2 and Ezh2 expression in mammary glands from 16 week-old NIC/Ezh2^+/+ and NIC/Ezh2^L/L mice. c IF analysis of ErbB2 and Ki67 expression in mammary glands from 16 week-old NIC/Ezh2^+/+ and NIC/Ezh2^L/L mice. d Quantification of Ki67-positive nuclei in ErbB2-expressing cells in mice of both genotypes. Data in (b–d) represent analyses of 4 independent mice per genotype, 5 fields of view per mammary gland. All scale bars represent 50 µm. e Hematoxylin-stained, whole-mounted mammary glands from MMTV-NIC mice treated for 10 weeks with GSK126 or vehicle control. White arrows indicate transformed mammary epithelial lesions. Scale bar indicates 500 µm. f IF analysis of ErbB2 and Ki67 expression in mammary glands from MMTV-NIC mice treated for 10 weeks with GSK126 or vehicle control. Scale bar indicates 100 µm. g Quantification of Ki67-positive nuclei in ErbB2-expressing cells in mice as in (f) (n = 6 per treatment group). In all scatter plots, the center line indicates the mean and error bars are ±SEM, with * = p < 0.05 (unpaired, two-tailed Student’s t-test)