Figure 4.

Murine roseolovirus (MRV) can be reactivated by minor histocompatibility antigen (miHA)-mismatched bone marrow transplant (BMT). (A) Schematic of experimental timeline. Newborn BALB/c (H2^d) mice were infected with MRV via intraperitoneal injection within 24 hours after birth. Mice were allowed to grow for 8–10 weeks to establish latent infection before receiving miHA-mismatched BMT by transplanting 5 × 10⁶ BM cells and 5 × 10⁶ splenocytes isolated from B10.D2 (H2^d) mice. Lung tissues were collected at 8–10 weeks of age (non-BMT control animals) or 1–8 weeks after BMT. (B) Expression levels of viral lytic gene pol in lung tissue after miHA-mismatched BMT. Each symbol represents a unique mouse. Dot plots are presented with mean ± SEM. *P < 0.05. (C) Expression levels of pol in various organs at 8 weeks after BMT (n = 5). The expression levels of pol are normalized to the expression of host GAPDH. Each symbol represents a unique mouse. Dot plots are presented with mean ± SEM. *P < 0.05. (D) Percentage of weight change in mice after miHA-mismatched BMT. Data are representative of three replicate experiments with five mice per group. Error bars indicate SEM.