Skip to main content
. 2019 Jun 25;10:770. doi: 10.3389/fphys.2019.00770

FIGURE 5.

FIGURE 5

Earlier and stronger fibrosis development in WT animals fed with Western diet containing non-trans fat shortening as fat source. (A) mRNA expression levels of IL-6 and TNF-α. Whole liver homogenates of WT animals after 24 weeks treatment with Western diets containing different fat sources (WD-Std, WD-NTF, WD-Corn) were analyzed via real-time PCR. 4 animals per group were included (∗p < 0.05). (B) Analysis of mRNA expression levels of collagen1α and α-SMA in whole liver homogenates. WT mice were fed WD (WD-Std, WD-NTF, WD-Corn) for 24 weeks. mRNA expression was analyzed via real-time PCR. 4 animals per group were included. (C) Representative Sirius Red stained liver sections of WT animals fed with steatosis inducing Western diets (WD-Std, WD-NTF, WD-Corn). (D) Quantitative evaluation of Sirius Red stained liver sections of WD treated animals (n = 4) (∗p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001). (E) α-SMA Western Blot analysis from whole liver extract of WT mice after treatment with WDs containing different fat sources (WD-Std, WD-NTF, WD-Corn). GAPDH serves as housekeeping protein. (F) Quantitative evaluation of α-SMA Western Blot analysis normalized to GAPDH expression levels. Analysis reveal a significant increase in protein levels of α-SMA in mice fed with WD containing non-trans fat shortening as fat source (n = 4) (∗∗p < 0.01).