Fig. 2.

BMP15 induces FSHR expression in HGrC1. a HGrC1 cells were treated 0–500 ng/ml BMP15 for 48 h. BMP15 induced FSHR mRNA expression in a concentration-dependent manner. b ChIP-qPCR: five histone (H3K9, H3K14, H4K8, H4K12, and H4K16) modifications were compared in HGrC1 cells with or without 250 ng/ml BMP15 for 24 h. Acetylation at H3K9 was significantly elevated with BMP15. c ChIP-qPCR: USF1 and USF2 binding to the FSHR promoter region were analyzed in HGrC1 cells with or without 250 ng/ml BMP15 for 24 h. Both transcriptional factor binding were significantly elevated with BMP15 stimulation. Data are shown as mean ± SD of three experiments. * p < 0.05