Table 2.
Replication of the feline adenovirus in different human (Homo sapiens) cell lines.
| Type of cells | Origin of cells | Cell line | Cytopathic effect | Viral antigen |
|---|---|---|---|---|
| Fibroblasts | Lung fibroblast | M426 (ATCC PTA-5244) | CPE+ | IFA+ |
| Primary human foreskin fibroblasts | – | CPE+ | IFA+ | |
| Epithelial cells | Cervical cancer | HeLa (ATCC CCL-2) | CPE+ | IFA+ |
| Embryonal kidney | 293 (ATCC CRL-1573) | CPE+ | IFA+ | |
| Breast adenocarcinoma | MCF7 (ATCC HTB-22) | CPE+ | IFA+ | |
| Melanoma | MEWO (ATCC HTB-65) | ∗CPE-/+ | ∗IFA-/+ | |
| Neural cells | Glioblastoma-astrocytoma | U87 (ATCC HTB-14) | CPE+ | IFA+ |
| Glioblastoma | A-172 (ATCC CRL-1620) | CPE+ | IFA+ | |
| Leukocytes | Human cord blood mononuclear cells (primary) | – | Degeneration, No CPE | IFA+ |
| Adult human peripheral blood lymphocytes (primary) | – | Degeneration, No CPE | IFA+ | |
| B-lymphocytes | Mature B-cell line | Bjab (DSMZ ACC 757) | No CPE | IFA+ |
| EBV transformed B-cell line | LCL- 8664 (ATCC CRL-1085) | No CPE | IFA+ | |
| Human histiocyte lymphoma | U937 (ATCC CRL-1593.2) | No CPE | IFA+ | |
| T-lymphocytes | Acute lymphoblastic leukemia | MOLT-3 (ATCC CRL-1552) | No CPE | IFA+ |
| Acute lymphoblastic leukemia | HSB-2 (ATCC CCL-120.1) | No CPE | IFA+ | |
| Lymphoblastic lymphoma | Sup-T1 (ATCC CRL-1942) | No CPE | IFA+ | |
| Acute T-cell leukemia | E6.1 (ATCC TIB-152) | No CPE | IFA+ |
ATCC, American type culture collection; ECACC, European collection of cell cultures; DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH/German collection of microorganisms and cell cultures; CPE, cytopathic effect; IFA, immunofluorescent antibody; IHC, immunohistochemistry. ∗MEWO, intracellular virus antigens and cytopathic effect developed from 7 days Postinfection.