FIGURE 2.

Lactate dehydrogenase releases were measured from primary neurons (A) and BV-2 microglia cells (B) in the experimental control group exposed to 1 h normoxia (21% O₂ in glucose-containing medium) and simulated ischemia group exposed to 1 h oxygen-glucose deprivation (OGD at 0.2% O₂ in glucose-depleted medium) at 37°C, and also after reperfusion from primary neurons (C) and BV-2 microglia cells (D) exposed to 1 h normoxia + 24 h reperfusion with complete medium (Normoxia/R) or 1 h OGD + 24 h reperfusion (OGD/R) at either 37 or 33.5°C. All CsA groups were pre-treated 1 h before experimental start with either 1 or 10 μM CsA at 37°C. Data from 4 to 6 individual experiments are presented as box-and-whiskers plot (box from 25th to 75th percentile and whisker min to max). Statistical analysis were conducted using one-way ANOVA followed by the Tukey post hoc test; ^∗∗p < 0.01, ^∗∗∗p < 0.001 compared to Normoxia at 37°C, and ^#p < 0.05 for group comparison were considered significant.