FIGURE 5.

Western Blot analysis was used to assess extracellular DAMPs, (A,D) heat shock protein 70 (Hsp70), (B,E) high mobility group box 1 (HMGB1), and (C) cold-inducible RNA-binding protein (CIRBP) released into the cultured supernatant from primary neurons and BV-2 microglial cells. The experimental control group was exposed to 1 h normoxia (21% O₂ in glucose-containing medium) and simulated ischemia group was exposed to 1 h oxygen-glucose deprivation (OGD at 0.2% O₂ in glucose-depleted medium) at 37°C. Quantitative densitometric analysis from 5 to 6 individual experiments is presented as box-and-whiskers plot (box from 25th to 75th percentile and whisker min to max), along with the representative immunoblots. Statistical analysis were conducted using one-way ANOVA followed by the Tukey post hoc test; ^∗∗p < 0.01, ^∗∗∗p < 0.001 compared to normoxia at 37°C, and ^#p < 0.05 for group comparison were considered significant.