Figure 7.

Disruption of nedd8 in zebrafish after SVCV infection increases viral replication and downregulates key antiviral genes. (A–C) Viral replication increased in nedd8-disrupted zebrafish larvae (nedd8^+/−&nedd8^−/−) larvae after SVCV infection, as compared to WT larvae. (D–G) The expression levels of key antiviral genes after SVCV infection were lower in nedd8-disrupted zebrafish larvae than in WT larvae. At 3 dpf, we added viruses (2 × 10⁸ TCID₅₀/ml) into the water containing zebrafish larvae. After incubation for 24 h, we extracted total RNA from all larvae and performed qPCR assays to detect the expression levels of ifn1 (D), mxc (E), and pkz (F), and ita (G). Data are shown as mean ± SEM of three independent experiments, each performed in triplicate; the statistical analysis was performed using GraphPad Prism 5 (unpaired t-test).