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. 2019 Jun 25;10:1432. doi: 10.3389/fimmu.2019.01432

Figure 9.

Figure 9

The antiviral response of nedd8-null adult zebrafish after SVCV infection is weaker than that of the WT zebrafish. (A–D) The key antiviral genes, ifn1 (A), mxc (B), pkz (C), and lta (D), downregulated in the kidneys of nedd8-null adult zebrafish (3 mpf; 0.38 ± 0.02 g), as compared to WT zebrafish (3 mpf; 0.38 ± 0.02 g). (E–H) The key antiviral genes, ifn1 (E), mxc (F), pkz (G), and lta (H), were downregulated in the spleens of nedd8−/− adult zebrafish (3 mpf; 0.38 ± 0.02 g), as compared to WT zebrafish (3 mpf; 0.38 ± 0.02 g). WT (nedd8+/+) and nedd8 −/− zebrafish were each i.p. injected with 10 μL SVCV (2 × 108 TCID50/ml). At 2 days post-injection (dpi), we extracted total RNA from the kidneys and spleens of all zebrafish and performed qPCR assays to determine the expression levels of ifn1, mxc, pkz, and lta. Data are shown as mean ± SEM of three independent experiments, each performed in triplicate; the statistical analysis was performed using GraphPad Prism 5 (unpaired t-test).