Table 1.
Primers used in RT-qPCR analysis.
| Gene name | Gene functions | Primers used in the RT-qPCR |
|---|---|---|
| aflR | Pathway regulator | F: AGCACTACAAACACTGACCCAC R: CCAGCACCTTGAGAACGATAA |
| aflCa | Noranthrone monooxygenase, Norsolorinate-anthrone to norsolorinate | F: GCACCAATGGAGCCGTAT R: GCGGTGTTCGTAGCGTTC |
| aflA | FAS alpha subunit, Acetate to polyketide | F: CGTGAGGTCAAGGCATTCCT R: GACTTGGCCCCCCTTCTGT |
| aflS | Transcription enhancer, Pathway regulator | F: CCGAAGATTCCGCTTGGA R: TGAAGACATGCAGCAAAAGGA |
| aflO | O-methyltransferase B, dihydrodemethylsterigmatocystin to dihydrosterigmatocystin | F: TGCTGTGGCATCCATTCAAA R: GGACTGCGTCTTCCAAAAGG |
| aflD | NOR reductase, norsolorinic acid to averantin | ACTGCGACTCGGAAACTGATG TGCTCCTCCCGCAATGTC |
| aflP | O-methyltransferase A, sterigmatocystin to O-methylsterigmatocystin | F: TGTGTCGAGTGATGTGGGACTAG R: GCCACCCAGCTCAACCTACA |
| aflF | NOR dehydrogenase, norsolorinic acid to averantin | F: AAGATGCTGGGCACGTTTG R: CATGGGTGAGGACGAATTGG |
| aflQ | Oxydoreductase, O-methylsterigmatocystin to AFB1 and AFG1, dihydro-Omethylsterigmatocystin to AFB2 and AFG2 | F: TTGCTGGGCTTGTGGATTC R: GAGGAGGACGCGTGTCTTTG |
| aflC | polyketide synthases, Acetate to polyketide | F: TCACAAGCGATGCACAGTTG R: AACTGACGAATGTGGGTCTTGTACT |
| aflX | Monooxygenase/oxidase, VA to DMST | F: ACCGCGTTGCACATCGT R: TGGGTGTCCACAACCTTCGT |
| AccC | Acetyl-CoA carbosylase, Acetyl-CoA to Malonlyl-CoA | F: ATGGTAAGACCTGCCTGCTA R: AGCGAGGATACCGAGGAT |
| β-tubulin | Endogenous control, Reference gene | F: AGCAGGCGAAGAAGGAGG R: ACGCCACGCATTTGATCTTC |