Table 1.

Effects of combination treatment on inhibition of A. fumigatus (NCPF2010) invasion.

Compartment Treated		Days (% Inhibition)
Upper	Lower	1	2	3	4	5	6	7	8
PC945 (0.1 μg/mL)	DMSO	86.4 ± 4.47d	3.36 ± 1.26	3.65 ± 0.38	4.47 ± 1.27	0.30 ± 0.59	−0.31 ± 2.28	0.90 ± 1.65	2.76 ± 1.43
DMSO	Posaconazole (0.01 μg/mL)	90.5 ± 2.79d	15.0 ± 0.96d	4.14 ± 0.82b	1.10 ± 0.63	−0.55 ± 1.86	−1.13 ± 2.49	0.25 ± 0.47	0.82 ± 0.28
PC945 (0.1 μg/mL)	Posaconazole (0.01 μg/mL)	98.9 ± 0.03d	99.1 ± 0.06d	97.8 ± 0.81d	91.0 ± 2.56d	64.1 ± 4.79c	38.2 ± 8.50	2.13 ± 1.5	3.73 ± 1.29
	SRa	1.00 ± 0.00	5.57 ± 0.33	13.0 ± 1.16	20.6 ± 7.70	136 ± 85.6	97.2 ± 87.4	5.61 ± 5.06	1.61 ± 0.63
Posaconazole (0.1 μg/mL)	DMSO	88.4 ± 2.89d	9.59 ± 3.78b	1.13 ± 0.51	−0.26 ± 1.41	−1.16 ± 1.33	0.34 ± 1.50	−0.06 ± 0.57	1.85 ± 1.70
DMSO	Posaconazole (0.1 μg/mL)	95.8 ± 0.61d	99.1 ± 0.03d	99.2 ± 0.00d	99.1 ± 0.07d	97.5 ± 1.29d	83.4 ± 9.94d	−4.24 ± 1.25	17.2 ± 2.38
Posaconazole (0.1 μg/mL)	Posaconazole (0.1 μg/mL)	95.6 ± 0.69d	99.2 ± 0.00d	99.2 ± 0.00d	99.2 ± 0.00d	99.0 ± 0.12d	97.1 ± 0.84d	9.04 ± 8.24	12.3 ± 2.96
	SRa	0.96 ± 0.01	1.00 ± 0.00	1.00 ± 0.00	1.00 ± 0.00	1.02 ± 0.01	1.20 ± 0.16	16.4 ± 7.87	0.64 ± 0.05
Voriconazole (1 μg/mL)	DMSO	95.0 ± 0.23d	88.8 ± 6.04d	59.4 ± 24.7b	30.8 ± 31.0	−1.61 ± 1.04	−1.67 ± 2.31	−0.55 ± 1.73	3.81 ± 1.71
DMSO	Voriconazole (1 μg/mL)	95.9 ± 0.374	99.1 ± 0.03d	99.2 ± 0.07d	94.8 ± 2.19d	−1.82 ± 1.01	−0.67 ± 2.92	6.92 ± 5.06	4.37 ± 3.31
Voriconazole (1 μg/mL)	Voriconazole (1 μg/mL)	95.5 ± 0.00d	99.1 ± 0.03d	99.3 ± 0.07d	94.9 ± 2.13d	−3.13 ± 0.20	−1.93 ± 3.84	7.74 ± 4.98	3.33 ± 2.16
	SRa	0.96 ± 0.00	0.99 ± 0.00	1.00 ± 0.00	0.98 ± 0.05	0.42 ± 0.08	0.63 ± 0.13	1.34 ± 0.59	0.41 ± 0.07

N = 3 of independent experiments, each experiment was conducted in duplicate.

^aSynergic Ratio (>1: Synergy), The value of 0.1 was applied for 0 or negative values to assist in the calculations.

^bp < 0.05, ^cp < 0.01, ^dp < 0.001 vs. infection control.