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. 2019 Jun 25;6:201. doi: 10.3389/fvets.2019.00201

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Copyright © 2019 Richards, Fothergill, Bernardeau and Wigley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative abundance of Bacteroides (A), Lachnospiraceae-Ruminococcaceae (B), Bacillus (C), Bifidobacteria (D), Clostridium Cluster IV (E), and Clostridium Cluster XIVa&b (F) in the mucus and lumen microbiota between 14 and 42 days post hatch. Data from different breeds was combined. Bacteroides shows a high affinity for the lumen while Bacillus is found almost exclusively in the mucus. A non-specific Lachnospiraceae-Ruminococcaceae primer pair showed consistently higher abundance in the mucus as do the more specific primers for Clostridium Cluster IV and XIVa&b. Bifidobacterium appears more abundant in the mucus; however this is not consistent across time points.