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. 2019 Jun 25;6:201. doi: 10.3389/fvets.2019.00201

Table 2.

Primer pairs used for quantitative PCR.

Target taxa Primers Amplicon size (b.p.) References
Domain Bacteria (targets V4 region) F: TGCCAGCMGCCGCGGTAA 254 (30)
R: GGACTACHVGGGTWTCTAAT
Bacteroides F: CCTWCGATGGATAGGGGTT 131 (31)
R: CACGCTACTTGGCTGGTTCAG
Lachnospiraceae-Ruminococcaceae F: CGGTACCTGACTAAGAAGC 429 (32)
R: AGTTTYATTCTTGCGAACG
Bacillus F: GCATTGGAAACTGGGGGACT 90 This study
R: CCGGTGTTCCTCCACATCTC
Bifidobacterium F: CTCCTGGAAACGGGTGG 550 (33)
R: GGTGTTCTTCCCGATATCTACA
Clostridium cluster IV F: TTACTGGGTGTAAAGGG 580 (34)
R: TAGAGTGCTCTTGCGTA
Clostridium cluster XIV a&b F: AAATGACGGTACCTGACTAA 438-441 (35)
R: CTTTGAGTTTCATTCTTGCGAA