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. 2019 Jun 25;10:1428. doi: 10.3389/fmicb.2019.01428

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Copyright © 2019 Watzer, Spät, Neumann, Koch, Sobotka, Macek, Hennrich and Forchhammer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proteins enriched in anti-FLAG-tag P_II immunoprecipitates from urea growth conditions. (Left) Scatterplot of the log₂ transformed LFQ protein ratios (P_II/control pull-down experiment) of 110 quantified proteins detected in two independent replicates. P_II pull-down specific outliers which are significantly enriched in both replicates are indicated in red, and significant outliers enriched in only one replicate are indicated in magenta (Replicate 1) or orange (Replicate 2) (p-value = 0.01). (Right) Heat map representation of the P_II-specific enrichment of nitrogen metabolism related proteins. Log₂ transformed LFQ protein ratios from both replicates are color coded and stars indicate significance. Proteins with missing LFQ ratios, detected exclusively in the P_II but not in the control pull-down, are striped in red/gray, and n.d. indicates no detection in P_II and control pull-down.