Table 1.
Oligonucleotides used in this study.
| Primer | Sequence (5′–3′ direction) |
|---|---|
| pKT25n_fw | ACCATGCAGCAATCGCATCAG |
| pKT25n_rev | CATAGCTGTTTCCTGTGTGAAATTG |
| glnB_fw | TGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGAAAAAAGTAGAAGCGATTATTC |
| glnB_rev | CTCGCTGGCGGCTGAATTCGAGCTCGGTACCCGGGGATCAATAGCTTCGGTATCCTTTTC |
| pipX_fw | TTCACACAGGAAACAGCTATGAGTAACGAAATTTACCTTAAC |
| pipX_rev | GATGCGATTGCTGCATGGTAAAAGTGTTTTTATGTAACTTTG |
| pipX_pKT25n_fw | AAGTTACATAAAAACACTTTTACCATGCAGCAATCGCATCAG |
| pipX_pKT25n_rev | TAAGGTAAATTTCGTTACTCATAGCTGTTTCCTGTGTGAAATTG |
| amt1_pKT25_fw | CTGGCGCGCACGCGGCGGGCTGCAGGGTCGACTCTAGAGATGTCTAATTCGATATTGTCTAAAC |
| amt1_pKT25_rev | AAAACGACGGCCGAATTCTTAGTTACTTAGGTACCCGGGGATCTTATTCAGGGACAGTGG |
| amt1_fw | AACAATTTCACACAGGAAACAGCTATGTCTAATTCGATATTGTCTAAAC |
| amt1_rev | TGATGCGATTGCTGCATGGTTTCAGGGACAGTGGCACCG |
| amt1_pKT25n_fw | TCTCCGGTGCCACTGTCCCTGAAACCATGCAGCAATCGCATC |
| amt1_pKT25n_rev | ACAATATCGAATTAGACATAGCTGTTTCCTGTGTGAAATTGTTATCCGC |
| nrtC_pKT25_fw | CGCGCACGCGGCGGGCTGCAGGGTCGACTCTAGAGGATCCCCCCTTCATTGAAATTGATCATGTTG |
| nrtC_pKT25_rev | AGTCACGACGTTGTAAAACGACGGCCGAATTCTTAGTTATTGATTAACTTGATCAATTTGGTCGATGAG |
| nrtC_fw | AATTTCACACAGGAAACAGCTATGCCCTTCATTGAAATTGATCATG |
| nrtC_rev | CTGATGCGATTGCTGCATGGTTTGATTAACTTGATCAATTTGG |
| nrtC_pKT25n_fw | AAATTGATCAAGTTAATCAAACCATGCAGCAATCGCATCAG |
| nrtC_pKT25n_rev | TCAATTTCAATGAAGGGCATAGCTGTTTCCTGTGTGAAATTG |
| nrtD_pKT25_fw | CGCACGCGGCGGGCTGCAGGGTCGACTCTAGAGGATCCCCAAACAATGAATGTCAATGACCCTATCC |
| ntrD_pKT25_rev | CCCAGTCACGACGTTGTAAAACGACGGCCGAATTCTTAGTTAAGACCCTTCCATGGATTCCACTGAGGGGGTAG |
| nrtD_fw | AATTTCACACAGGAAACAGCTATGCAAACAATGAATGTCAATGACCCTATC |
| nrtD_rev | CTGATGCGATTGCTGCATGGTAGACCCTTCCATGGATTCCACTGAG |
| nrtD_pKT25n_fw | TGGAATCCATGGAAGGGTCTACCATGCAGCAATCGCATCAG |
| nrtD_pKT25n_rev | ATTGACATTCATTGTTTGCATAGCTGTTTCCTGTGTGAAATTG |
| urtD_pKT25_fw | CGCACGCGGCGGGCTGCAGGGTCGACTCTAGAGGATCCCACCAGCAAAATCTTAGAAATTCAAG |
| urtD_pKT25_rev | CCCAGTCACGACGTTGTAAAACGACGGCCGAATTCTTAGCTAATCTCCATCCTCATCAAC |
| urtD_fw | ACACAGGAAACAGCTATGACCAGCAAAATCTTAGAAATTCAAGAC |
| urtD_rev | GATGCGATTGCTGCATGGTATCTCCATCCTCATCAACACTG |
| urtD_pKT25n_fw | AGTGTTGATGAGGATGGAGATACCATGCAGCAATCGCATCAG |
| urtD_pKT25n_rev | TTCTAAGATTTTGCTGGTCATAGCTGTTTCCTGTGTGAAATTG |
| urtE_fw | GCGCGCACGCGGCGGGCTGCAGGGTCGACTCTAGAGGATGCTATGTTATCCTTTCCCCCATTCTTG |
| urtE_rev | CCCAGTCACGACGTTGTAAAACGACGGCCGAATTCTTAGTTATACTGCCAAAAATTTTTGGATAAC |
| urtE_fw | ACAATTTCACACAGGAAACAGCTATGGCTATGTTATCCTTTCCC |
| urtE_rev | TGATGCGATTGCTGCATGGTTACTGCCAAAAATTTTTGGATAACC |
| urtE_pKT25n_fw | TATCCAAAAATTTTTGGCAGTAACCATGCAGCAATCGCATCAG |
| urtE_pKT25n_rev | GGGAAAGGATAACATAGCCATAGCTGTTTCCTGTGTGAAATTG |