Table 2.
Plasmids used in this study.
| Plasmid | Tag localization | Description | References |
|---|---|---|---|
| pPD-NFLAG | N-terminal | Encoding the N-terminal 3xFLAG tag | Hollingshead et al., 2012 |
| pPD-CFLAG | C-terminal | Encoding the C-terminal 3xFLAG tag | Chidgey et al., 2014 |
| pKT25 | Encoding T25 fragment of adenylate cyclase CyaA (amino acids 1–224) | Karimova et al., 2001 | |
| pKT25n | Derived from pKT25. Upstream of the T25 fragment | This study | |
| pUT18 | Encoding T18 fragment of adenylate cyclase CyaA (amino acids 225–399) | Karimova et al., 2001 | |
| pUT18 glnB | N-terminal | Derived from pUT18. Encoding glnB | This study |
| pUT18 glnB (I86N) | N-terminal | Derived from pUT18. Encoding glnB containing the I86N mutation | This study |
| pKT25n pipX | C-terminal | Derived from pKT25. Sequence encoding pipX. Positive control | This study |
| pKT25 pipX | N-terminal | Derived from pKT25. Sequence encoding pipX. Positive control | This study |
| pKT25n amt1 | C-terminal | Derived from pKT25. Sequence encoding amt1 | This study |
| pKT25 amt1 | N-terminal | Derived from pKT25. Sequence encoding amt1 | This study |
| pKT25n nrtC | C-terminal | Derived from pKT25. Sequence encoding nrtC | This study |
| pKT25 nrtC | N-terminal | Derived from pKT25. Sequence encoding nrtC | This study |
| pKT25n nrtD | C-terminal | Derived from pKT25. Sequence encoding nrtD | This study |
| pKT25 nrtD | N-terminal | Derived from pKT25. Sequence encoding nrtD | This study |
| pKT25n urtD | C-terminal | Derived from pKT25. Sequence encoding urtD | This study |
| pKT25 urtD | N-terminal | Derived from pKT25. Sequence encoding urtD | This study |
| pKT25n urtE | C-terminal | Derived from pKT25. Sequence encoding urtE | This study |
| pKT25 urtE | N-terminal | Derived from pKT25. Sequence encoding urtE | This study |