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. 2019 Jun 25;13:276. doi: 10.3389/fncel.2019.00276

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Copyright © 2019 Wang, Liao, Huang, Wang, Zhou, Chen, Liu, Ji, Xia, Jiang, Huang and Xiong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calcium concentrations in retinal neuron cultures treated with 50 μM glutamate injury for different time points. (A) Calcium concentrations indicated by Fluo-4 signals. (B) Quantitative analysis of Fluo-4 fluorescence signal intensity. (C) The fold change of calcium concentrations was determined by cell calcium assay kit. Data were analyzed using one-way ANOVA. ^*p < 0.05 vs. CTL group. These data are representative of results from five independent experiments. Scale bar = 20 μm in all panels.