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. 2019 Jun 25;13:276. doi: 10.3389/fncel.2019.00276

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Copyright © 2019 Wang, Liao, Huang, Wang, Zhou, Chen, Liu, Ji, Xia, Jiang, Huang and Xiong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calcium concentrations in retinal neuron cultures after different GluRs inhibition. (A) Calcium concentrations indicated by Fluo-4 signals. (B) Quantitative analysis of Fluo-4 signals intensity. (C) The fold change of calcium concentrations was determined by cell calcium assay commercial kit. MK, CN, and MC, which respectively block NMDAR, AMPA/KA receptor and mGluRs, were added to the cultures at a final concentration of 10, 10, and 50 μM, 30 min before 15 min glutamate insult. Experiments were repeated three times. Data were analyzed using one-way ANOVA. ^*p < 0.05 vs. CTL group, ^# p < 0.05 vs. Glu group. Scale bar = 20 μm in all panels.