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. 2019 Jun 25;13:276. doi: 10.3389/fncel.2019.00276

FIGURE 5.

FIGURE 5

The regulatory role of CaMKII in Pin1 activity. (A) Three dimensional structure of proteins by homologous modeling (Swiss Model). (B) Protein surface electrostatic potential energy are calculated by APBS (Figures are produced by using PyMOL). (C) The initial binding mode is constructed by manually binding areas with opposite electrostatic potential of two proteins and then the protein complex is energy minimized using molecular dynamics software NAMD v2.12. (D) IP assay of CaMKII and Pin1. Pin1 was pull-down with CaMKII antibody suggested by a 18 KDa band as observed. (E) Western blot of CaMKII, p-CaMKII, and Pin1 expression after Glu, MC, BA, and KN treatment. (F) The statistical analysis of p-CaMKII/CaMKII expression. (G) The statistical analysis of Pin1 expression. MC, BA, and KN were used 30 min before glutamate treatment at a concentration of 50, 10, and 10 μM, respectively. N = 3 cultures. Data were analyzed using one-way ANOVA. *p < 0.05 vs. CTL group, #p < 0.05 vs. Glu group.