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. 2019 Jun 25;13:276. doi: 10.3389/fncel.2019.00276

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Copyright © 2019 Wang, Liao, Huang, Wang, Zhou, Chen, Liu, Ji, Xia, Jiang, Huang and Xiong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Glutamate-induced necrosis decreased by KN treatment. (A) Retinal necrotic neurons were stained with PI (red). Nuclei were counterstained with DAPI (blue). (B) Statistical analysis of PI-positive retinal neurons. (C) The percentage of necrotic neurons after glutamate treatment and pretreated with 50 μM MC, 10 μM BA, and 10 μM KN was determined by LDH release assay. N = 3 cultures. Data were analyzed using one-way ANOVA. ^*p < 0.05 vs. CTL group, ^#p < 0.05 vs. Glu group. Scale bar = 20 μm in all panels.