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. 2019 Jun 25;10:794. doi: 10.3389/fpls.2019.00794

Figure 1.

Figure 1

Phylogenetic analysis of the prenyltransferase family in citrus species and Lithospermum erythrorhizon as well as in model plants. (A) Grouping of plant prenyltransferases into three major clades: a clade represented by the Arabidopsis homogentisate phytyltransferase AtVTE2-1 involved in vitamin E biosynthesis (indicated by “Vitamin E” and a yellow background), a clade represented by Arabidopsis AtVTE2-2 for plastoquinone biosynthesis (indicated by “Plastoquinone” and a gray background), and a clade represented by the rice polyprenyltransferase OsPPT1 for ubiquinone biosynthesis (indicated by “Ubiquinone” and a compressed black triangle). Biochemically characterized proteins are indicated by a white background. The Citrus and legume proteins are shown in red and blue letters, respectively, and the lineage-specific clades are indicated by brackets with the same colors. (B) Details of the phylogenetic tree of polyprenyltransferases for ubiquinone in panel (A). L. erythrorhizon proteins are shown in magenta letters. The brackets indicate subclades of polyprenyltransferases involved in ubiquinone biosynthesis (PPT subfamily), LePGT-like proteins (LePGT subfamily), and unclassified subclade proteins (unclassified). The proteins from other organisms are shown in black letters. The asterisk indicates the putative PPT-like protein of L. erythrorhizon. The phylogenetic tree was drawn using the MEGA7 neighbor-joining method with 1,000 bootstrap replicates for alignment of polyprenyltransferase-related proteins, which were calculated with the MUSCLE algorithm. The accession numbers are shown next to the name of the organism. Biochemically characterized proteins are indicated by a yellow background. The scale bar represents 0.1 amino acid substitutions per site. (C) LePGT gene is encoded by a single exon gene in the L. erythrorhizon genome, whereas LePPT-like proteins are encoded by genes with inserted introns, at positions similar to those of the authorized OsPPT gene and the closest tobacco homolog, NtPPT-like gene (gene=LOC107804153). The first intron insertion site into the coding region is shown. Scale bar, 1kb of DNA sequence. Blue boxes represent coding exons.