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. 2019 Jul 1;9:9428. doi: 10.1038/s41598-019-45878-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viral protein burden in EcoHIV-1 infected mice. Adult C57BL/6 mice were administered IP, 30 min prior to IC unilateral injection of 1 × 10⁶ pg p24 of EcoHIV-1 with rosiglitazone (10 mg/kg/day) or pioglitazone (20 mg/kg/day). Cytoplasmic fractions of subcortical brain regions were isolated 5 days post inoculation and p24 protein expression was measured by Wes. β-actin was used as a loading control. (a) Representative blot which was cropped for clarity and (b) densitometric analysis. Full-length blots are presented in Supplementary Fig. 6S. Results are expressed as mean ± SEM; n = 3–5 animals/group. Asterisks symbol represents data points significantly different from EcoHIV-1 (vehicle) respectively (*p < 0.05, **p < 0.01).