Figure 2. Design of expression constructs containing the chain elongation pathway of A. thaliana.

(A) The two constructs used in combination to express the biosynthetic genes. The MAM gene is replaced with native or mutated variants of MAM1 and MAM3 as well as IPMS2. Different T7 promoters were used as indicated in the arrows. Ribosomal binding sites, genes and terminators are indicated as dark grey, light grey and black boxes, respectively. (B) Promoters used in this work. Sequence differences are highlighted.