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. 2019 Jul 2;39(7):BSR20190446. doi: 10.1042/BSR20190446

Figure 4. Levels of protein amino acid substrates in E. coli expressing the chain elongation pathway with different MAM variants.

Figure 4

GA, GAG and GAM represent MAM variants with multiple mutations; GA, T257G/G259A; GAG, T257G/G259A/A290G; GAM, T257G/G259A/L186M. Levels of leucine, phenylalanine and valine are statistically unchanged between the constructs when comparing to the average mean of all cultures. Methionine levels vary between cultures, although the differences are small. Data represent average and standard deviation of three biological replicates each grown in three technical replicates.