Figure 6.

Overexpression of miR-506 inhibits proliferation, migration and invasion of colorectal cancer cells. HCT116 cells were transfected with 75 nM miR-NC (or miR-506 mimics). A. CCK-8 assay was used to assess cell viability daily, on Days 1-3 after transfection. B. At 48 h after transfection, cells were harvested and fixed in cold 70% ethanol at 4℃ overnight, then cell cycle distribution was determined via flow cytometry. C. 2000 transfected cells were inoculated in 6-well plates and cultured for 2 weeks, then colonies with >50 cells were counted under a microscope. D and E. Transwell migration (D) and invasion (E) assays were used to elucidate the migration and invasion of cells, respectively. ImageJ software was used for cell counting. All error bars represent the SEM from at least 3 independent experiments. **p<0.01 vs. miR-NC.