Figure 6.

Effect of Vitamin E on TNF‐α activated NF‐κB expression. The (a) MDA‐MB‐231 and (b) MCF‐7 human breast cancer cells were co‐cultured for 24 h with 10 μg/ml of various isomers of vitamin E. These cells were briefly exposed to 1 nM TNF‐α for 30 min. Nuclear extracts were prepared and analysed for NF‐κB expression by ELISA. Results are shown as the mean ± STD from duplicate cultures. (*) values are significantly different from the control +TNF group, (#) values are significantly different from the control group, p < 0.05 (one‐way ANOVA).