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. 2019 Jun 28;25(24):3030–3043. doi: 10.3748/wjg.v25.i24.3030

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©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Detection of alpha-fetoprotein and glypican-3 antigen expression in Hepa1-6/GPC3 cells by flow cytometry. Flow cytometry data showed significantly higher alpha-fetoprotein expression in the cytoplasm (A) than in the membrane (B), compared with blank cell and IgG isotype controls. C: The positive shift of fluorescence distribution compared with isotype control illustrated higher membrane expression of the glypican-3 antigen. AFP: Alpha-fetoprotein; GPC3: Glypican-3.