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. 2019 May 9;44:311–321. doi: 10.1016/j.ebiom.2019.05.003

Fig. 4.

Fig. 4

miR-567 negatively regulates with PI3K/AKT-c-Myc signalling in GC. (A) GSEA demonstrated enrichment of PI3K-AKT-mTOR signalling pathway in high PIK3AP1 expression GC group. (B) Western blot experiments were used to analyse the expression of relevant proteins in PI3K/AKT signal pathway after PIK3AP1 overexpression in MGC803 and BGC823 cells. (C) Western blot experiments were used to analyse the expression of relevant proteins in PI3K/AKT signal pathway after miR-567 knockdown and overexpression in MGC803 and BGC823 cells. (D) Western blot experiments were used to analyse the expression of relevant proteins in PI3K/AKT signal pathway in LV-miR-567-transfected cells and LV-miR-NC-transfected cells. (E) The upregulation of p-AKT and c-Myc induced by anti-miR-567 were abrogated after administration of LY294002 in MGC803 and BGC823 cells. (F) The downregulation of p-AKT and c-Myc induced by introduction of PIK3AP1 were abrogated after administration of miR-567 in MGC803 and BGC823 cells.