Fig. 2.

NH₃Induced MMP Depolarization. (A) MCEC (WT and Slc4a11 KO) were loaded with 30 nM TMRE for 30 min then perfused in a HEPES buffered ringer with 10 nM TMRE. After stabilization 1, 10, or 25 mM NH₄Acetate was added. Wild type responses (blue and black traces labelled 1, 10, and 25) showed decreased fluorescence (ΔF/F) indicating depolarization of MMP while KO cells (red traces) showed little depolarization at any [NH₄Acetate]. Bar graph indicates total TMRE fluorescence change and rate of change, n = 3, *p < 0.05. (B) Endothelial cell surface of WT and KO corneas from 12 week old mice were loaded with 30 nM TMRE for 30 min and perfused with 10 nM TMRE. After stabilization, corneas were exposed to 25 mM NH₄Acetate.Top-Average time trace of TMRE fluorescence, Bottom bar graph- Maximum change, n = 3, *p < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)