Detrimental Effect of NH3on Apoptosis and MitoROS in MCEC KO cells. (A) Apoptosis vs. [Ammonium Acetate] in Gluc 1 g/L for 24 h, n = 3, *greater than WT p < 0.05. (B) Percentage of MitoSOX+ cells following 24 h incubation with 25 mM NH4Acetate, n = 3 *p < 0.05 vs Gluc; same genotype. (C) Effect of 24 h of 25 mM NH4Acetate on %TMRE+ cells; effect of 0.5 mM Gln shown for comparison, n = 3, *p < 0.05 vs Gluc, same genotype. (D) Effect of 24 h of 25 mM NH4Acetate on [ATP], n = 3, *p < 0.05 vs Gluc, same genotype.