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. 2019 May 6;146(8):1075–1082. doi: 10.1017/S0031182019000441

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© Cambridge University Press 2019

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Tcgp63-I expression in T. cruzi Dm28c is enhanced after passage in R. prolixus. Cells were re-isolated in culture after insect colonization, as described in materials and methods. Long-term culture-adapted parasites (white) and recently isolated parasites (grey) were incubated in the presence of anti-Tcgp63-I antibody at 1:500 dilution and analysed by flow cytometry. Representative data of the analysis of 10 000 cells from one of three experiments are shown. The inset shows the mean of fluorescence intensity (MFI) levels. The asterisk indicates different values for MFI level between control and trypomastigotes re-isolated (*P < 0.05).