Fig. 4.

Chem-seq shows cluster-dependent signal profiles of BRD4/I-BET151 interactions. a Method scheme featuring loci recovered by ChIP-seq alone and those recovered by ChIP-seq and Chem-seq. Using an antibody against a BET protein member, we can recover all genomic loci bound by the protein. Using a biotinylated derivative of I-BET121, we can pull down fragments where the BET protein exhibits an accessible bromodomain. b Normalized genome-wide cluster-specific Chem-seq profiles on TSS −/+ 4 Kb in the absence or presence of excess free I-BET151 as control