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. 2019 Jul 1;18:118. doi: 10.1186/s12934-019-1168-z

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of UDP-Glc formation. a The supernatant of reaction catalyzed by the hybrid cascade system comprised of separate whole cell catalysts expressing TmαGP and StUSP, in the optimized conditions described above, was assayed by PAMN-HPLC. b ESI–MS spectrum of product UDP-Glc. The supernatant of reaction catalyzed by the hybrid cascade system comprised of separate whole cell catalysts expressing TmαGP and StUSP, in the optimized conditions described above, was assayed by MS after desalting using a P2 column