Table 2.
Plasmids and strains used in this study
| Strain or plasmid | Characteristics | Source |
|---|---|---|
| E. coli strains | ||
| DH5α | Thermo Fisher | |
| BL21 (DE3) | Thermo Fisher | |
| BL21-TmαGP | pET20b-TmαGP | This work |
| BL21-StUSP | pET21b-StUSP | This work |
| BL21-PiUDH | pET15b-PiUDH | This work |
| BL21-TkNOX | pET21b-TkNOX | This work |
| BL21-PiUDH-TkNOX | pET15b-PiUDH and pACYCDuet1-TkNOX | This work |
| Plasmids | ||
| pET20b(+) | Expression vector, ApR | Novagen |
| pET21b(+) | Expression vector, ApR | Novagen |
| pET15b | Expression vector, ApR | Novagen |
| pACYCDuet-1 | Expression vector, CmR | Novagen |
| pET20b-TmαGP | Expression vector of TmαGP | This work |
| pET21b-StUSP | Expression vector of ST0452 | This work |
| pET15b-PiUDH | Expression vector of PiUDH | This work |
| pET20b-TkNOX | Expression vector of TkNOX | This work |
| pACYC Duet1-TkNOX | Expression vector of TkNOX | This work |