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. 2019 Jul 1;12:371. doi: 10.1186/s13104-019-4412-z

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — a Amplification curves obtained over five orders of magnitude. From the left to the right, the curves represent five tenfold serial dilutions (performed in triplicates) and three twofold dilutions (performed in 20 replicates), from 4.23 to 0.025 lg TCD50/mL, of DNA. b Linearity of the PCR assay results over five orders of magnitude. The equation of the standard curve obtained was y = − 3.4677 + 0.2864*x, and the determination coefficient r2 was 0.9997. Reactions of the no-template controls tested showed no Ct values (data not shown)