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. 2019 Jun 18;2019:6107456. doi: 10.1155/2019/6107456

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Copyright © 2019 Shivani Baisiwala et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Recurrent PDX GBM43 tumors contain elevated tumor-derived vessels. Recurrent PDX GBM43 was established as described. (a) Brains containing tumors were removed and subjected to immunofluorescence (IF) analysis for CD105 with an antibody specific to the human antigen, a marker of intermediate endothelial cells and laminin, which indicates functional vessels. Tumors were identified via DAPI nuclear staining. (b) IF staining for CD31, a marker of mature endothelial cells and laminin. Images taken at 20x magnification.