Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 17;2019:7604396. doi: 10.1155/2019/7604396

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Yujin Zhang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Inducible expression of sFRP1 in corneal stroma. (a) Diagram showing the generation of bitransgenic mouse strain keratocan rtTA/Tet-O-sFRP1 (KR/sFRP1). Bitransgenic mice were generated by breeding keratocan/rtTA knock-in (KR/rtTA) mice with tetracycline-On promoter-driven sFRP1 (Tet-O-sFRP1). Bitransgenic mice expressed sFRP1 in corneal stroma upon doxycycline induction. (b) Corneal stromal expression of reporter gene GFP in adult KR/GFP mice. (c, d) Immunostaining verified that only bitransgenic KR/sFRP1 mice were positive for sFRP1 in the corneal stroma. Abbreviations: cn = cornea, cj = conjunctiva, ep = epithelium, and st = stroma.